Unknown

Dataset Information

0

Metabolism of the Fusarium Mycotoxins T-2 Toxin and HT-2 Toxin in Wheat.


ABSTRACT: To investigate the metabolic fate of HT-2 toxin (HT2) and T-2 toxin (T2) in wheat (Triticum aestivum L.), an untargeted metabolomics study utilizing stable isotopic labeling and liquid chromatography-high resolution mass spectrometry was performed. In total, 11 HT2 and 12 T2 derived in planta biotransformation products were annotated putatively. In addition to previously reported mono- and diglucosylated forms of HT2, evidence for the formation of HT2-malonyl-glucoside and feruloyl-T2, as well as acetylation and deacetylation products in wheat was obtained for the first time. To monitor the kinetics of metabolite formation, a time course experiment was conducted involving the Fusarium head blight susceptible variety Remus and the resistant cultivar CM-82036. Biotransformation reactions were observed already at the earliest tested time point (6 h after treatment), and formed metabolites showed different kinetic profiles. After ripening, less than 15% of the toxins added to the plants were determined to be unmetabolized.

SUBMITTER: Nathanail AV 

PROVIDER: S-EPMC4570218 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature

altmetric image

Publications


To investigate the metabolic fate of HT-2 toxin (HT2) and T-2 toxin (T2) in wheat (Triticum aestivum L.), an untargeted metabolomics study utilizing stable isotopic labeling and liquid chromatography-high resolution mass spectrometry was performed. In total, 11 HT2 and 12 T2 derived in planta biotransformation products were annotated putatively. In addition to previously reported mono- and diglucosylated forms of HT2, evidence for the formation of HT2-malonyl-glucoside and feruloyl-T2, as well a  ...[more]

Similar Datasets

| S-EPMC4495206 | biostudies-literature
| S-EPMC5198558 | biostudies-literature
| S-EPMC7232504 | biostudies-literature
| S-EPMC10905990 | biostudies-literature
| S-EPMC6017960 | biostudies-literature
| S-EPMC7298049 | biostudies-literature
| S-EPMC7761704 | biostudies-literature
| S-EPMC5086661 | biostudies-literature
| S-EPMC3579838 | biostudies-other
| S-EPMC3720990 | biostudies-literature