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Dual-color 3D superresolution microscopy by combined spectral-demixing and biplane imaging.

ABSTRACT: Multicolor three-dimensional (3D) superresolution techniques allow important insight into the relative organization of cellular structures. While a number of innovative solutions have emerged, multicolor 3D techniques still face significant technical challenges. In this Letter we provide a straightforward approach to single-molecule localization microscopy imaging in three dimensions and two colors. We combine biplane imaging and spectral-demixing, which eliminates a number of problems, including color cross-talk, chromatic aberration effects, and problems with color registration. We present 3D dual-color images of nanoscopic structures in hippocampal neurons with a 3D compound resolution routinely achieved only in a single color.

SUBMITTER: Winterflood CM

PROVIDER: S-EPMC4571034 | biostudies-literature | 2015 Jul

REPOSITORIES: biostudies-literature

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Dual-color 3D superresolution microscopy by combined spectral-demixing and biplane imaging.

Winterflood Christian M CM Platonova Evgenia E Albrecht David D Ewers Helge H

Biophysical journal 20150701 1

Multicolor three-dimensional (3D) superresolution techniques allow important insight into the relative organization of cellular structures. While a number of innovative solutions have emerged, multicolor 3D techniques still face significant technical challenges. In this Letter we provide a straightforward approach to single-molecule localization microscopy imaging in three dimensions and two colors. We combine biplane imaging and spectral-demixing, which eliminates a number of problems, includin ...[more]

PMID: 26153696

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