Dataset Information

First report of Turnip mosaic virus occurrence in cole crops (Brssica spp) from Arunachal Pradesh, India.

ABSTRACT: The occurrence of Turnip mosaic virus (TuMV) in cole crops (Brassica spp) grown in Basar, Arunachal Pradesh, India was confirmed by symptomatology, transmission electron microscopy, reverse transcription-polymerase chain reaction and partial characterization of cytoplasmic inclusion protein and coat protein (CP) domains. Phylogenetic analysis of the partial CP sequences of the new TuMV isolates from Indian mustard (AR-IndM), broad leaved mustard (AR-BrLM) and broccoli (AR-Broc) revealed their closest relationship with members of the World-B genogroup of TuMV. This is the first molecular evidence of TuMV infection in Brassica spp from India.

SUBMITTER: Singh R

PROVIDER: S-EPMC4571583 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

First report of Turnip mosaic virus occurrence in cole crops (Brssica spp) from Arunachal Pradesh, India.

Singh Raghuveer R Banerjee Amrita A Sharma Susheel Kumar SK Bhagawati R R Baruah Sikimoni S Ngachan S V SV

Virusdisease 20150811 3

The occurrence of Turnip mosaic virus (TuMV) in cole crops (Brassica spp) grown in Basar, Arunachal Pradesh, India was confirmed by symptomatology, transmission electron microscopy, reverse transcription-polymerase chain reaction and partial characterization of cytoplasmic inclusion protein and coat protein (CP) domains. Phylogenetic analysis of the partial CP sequences of the new TuMV isolates from Indian mustard (AR-IndM), broad leaved mustard (AR-BrLM) and broccoli (AR-Broc) revealed their cl ...[more]

PMID: 26396991

Similar Datasets

Project description:Microbiota inhabiting midguts of mosquitoes play a key role in the host - parasite interaction and enhance vectorial capacity of viral diseases like dengue and chikungunya fevers. Mosquito midgut is considered to be an important site for host-pathogen interaction and pathogen survival is thought to be an outcome of this interaction. In the present study we examined the bacterial community in the midgut of Aedes mosquitoes in Arunanchal Pradesh, India, a subtropical zone where dengue fever is reported to be emerging.Larvae and pupa of Aedes mosquitoes were collected from a biodiversity hotspot, Bhalukpong, Arunachal Pradesh, India. 16S rRNA gene sequences were used for identification of isolated bacterial population from each species of mosquitoes. We used various diversity indices to assess the diversity and richness of the bacterial isolates in both mosquito species.On the basis of 16S rRNA gene sequence analysis a total of 24 bacterial species from 13 genera were identified belonging to 10 families of four major phyla. Phylum Proteobacteria was dominant followed by Firmicutes, Bacteroidetes and Actinobacteria. The midgut bacteria belonging to the phylum Proteobacteria and Firmicutes were isolated from both Ae. albopictus and Ae. aegypti, whereas, bacteria belonging to phylum Bacteroidetes and Actinobacteria were isolated only from Ae. albopictus and Ae. aegypti respectively. Enterobacter cloacae was the dominant bacterial species in both Ae. albopictus (33.65%) and Ae. aegypti (56.45%). Bacillus aryabhattai (22.78%) was the second most common bacterial species in Ae. albopictus whereas, in Ae. aegypti the second most common bacterial species was Stenotrophomonas maltophilia (7.44%).The family Enterobacteriaceae of phylum Proteobacteria was dominant in both species of Aedes mosquitoes. To the best of our knowledge, this is the first attempt to study midgut microbiota from a biodiversity hotspot in Northeastern India. Some bacterial genera Enterobacter and Acinetobacter isolated in this study are known to play important roles in parasite-vector interaction. Information on midgut microflora may lead towards the development of novel, safe, and effective strategies to manipulate the vectorial capacity of mosquitoes.

Dataset Information

First report of Turnip mosaic virus occurrence in cole crops (Brssica spp) from Arunachal Pradesh, India.

Publications

First report of Turnip mosaic virus occurrence in cole crops (Brssica spp) from Arunachal Pradesh, India.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets