Ontology highlight
ABSTRACT: Aim
To investigate the effect of histone acetylation on regulation of p21WAF1 gene expression in human colon cancer cell lines.Methods
Two cell lines, Colo-320 and SW1116 were treated with either trichostatin or sodium butyrate. Expressions of p21WAF1 mRNA and protein were detected by real-time RT-PCR and Western blotting, respectively. Acetylation of two regions of p21WAF1 gene-associated histones and total cellular histones were examined by chromatin immunoprecipitation assay and Western blotting.Results
Trichostatin or sodium butyrate re-activated p21WAF1 transcription resulted in up-regulated p21WAF1 protein level in colon cancer cell lines. Those effects were accompanied by an accumulation of acetylated histones in total cellular chromatin and p21WAF1 gene-associated region of chromatin.Conclusion
Histone acetylation regulates p21WAF1 expression in human colon cancer cell lines, Colo-320 and SW1116.
SUBMITTER: Chen YX
PROVIDER: S-EPMC4572185 | biostudies-literature | 2004 Sep
REPOSITORIES: biostudies-literature
Chen Ying-Xuan YX Fang Jing-Yuan JY Zhu Hong-Yin HY Lu Rong R Cheng Zhong-Hua ZH Qiu De-Kai DK
World journal of gastroenterology 20040901 18
<h4>Aim</h4>To investigate the effect of histone acetylation on regulation of p21WAF1 gene expression in human colon cancer cell lines.<h4>Methods</h4>Two cell lines, Colo-320 and SW1116 were treated with either trichostatin or sodium butyrate. Expressions of p21WAF1 mRNA and protein were detected by real-time RT-PCR and Western blotting, respectively. Acetylation of two regions of p21WAF1 gene-associated histones and total cellular histones were examined by chromatin immunoprecipitation assay a ...[more]