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Histone acetylation regulates p21WAF1 expression in human colon cancer cell lines.

ABSTRACT: AIM:To investigate the effect of histone acetylation on regulation of p21WAF1 gene expression in human colon cancer cell lines. METHODS:Two cell lines, Colo-320 and SW1116 were treated with either trichostatin or sodium butyrate. Expressions of p21WAF1 mRNA and protein were detected by real-time RT-PCR and Western blotting, respectively. Acetylation of two regions of p21WAF1 gene-associated histones and total cellular histones were examined by chromatin immunoprecipitation assay and Western blotting. RESULTS:Trichostatin or sodium butyrate re-activated p21WAF1 transcription resulted in up-regulated p21WAF1 protein level in colon cancer cell lines. Those effects were accompanied by an accumulation of acetylated histones in total cellular chromatin and p21WAF1 gene-associated region of chromatin. CONCLUSION:Histone acetylation regulates p21WAF1 expression in human colon cancer cell lines, Colo-320 and SW1116.

SUBMITTER: Chen YX

PROVIDER: S-EPMC4572185 | biostudies-literature | 2004 Sep

REPOSITORIES: biostudies-literature

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Histone acetylation regulates p21WAF1 expression in human colon cancer cell lines.

Chen Ying-Xuan YX Fang Jing-Yuan JY Zhu Hong-Yin HY Lu Rong R Cheng Zhong-Hua ZH Qiu De-Kai DK

World journal of gastroenterology 20040901 18

<h4>Aim</h4>To investigate the effect of histone acetylation on regulation of p21WAF1 gene expression in human colon cancer cell lines.<h4>Methods</h4>Two cell lines, Colo-320 and SW1116 were treated with either trichostatin or sodium butyrate. Expressions of p21WAF1 mRNA and protein were detected by real-time RT-PCR and Western blotting, respectively. Acetylation of two regions of p21WAF1 gene-associated histones and total cellular histones were examined by chromatin immunoprecipitation assay a ...[more]

PMID: 15309711

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Project description:BACKGROUND:Since the first evidence suggesting existence of stem-like cancer cells, the process of cells reprogramming to the stem cell state remains as an attractive tool for cancer stemness research. Current knowledge in the field of cancer stemness, indicates that the microenvironment is a fundamental regulator of cell behavior. With regard to this, we investigated the changes of genome wide gene expression in reprogrammed human colon normal epithelial CRL-1831 and colon carcinoma DLD1 cell lines grown under more physiologically relevant three-dimensional (3D) cell culture microenvironment compared to 2D monolayer. METHODS:Whole genome gene expression changes were evaluated in both cell lines cultured under 3D conditions over a 2D monolayer by gene expression microarray analysis. To evaluate the biological significance of gene expression changes, we performed pathway enrichment analysis using the Kyoto Encyclopedia of Genes and Genomes (KEGG) database. Gene network analysis was used to study relationships between differentially expressed genes (DEGs) in functional categories by the GeneMANIA Cytoscape toolkit. RESULTS:In total, we identified 3228 and 2654 differentially expressed genes (DEGs) for colon normal and cancer reprogrammed cell lines, respectively. Furthermore, the expression of 1097 genes was commonly regulated in both cell lines. KEGG enrichment analysis revealed that in total 129 and 101 pathways for iPSC-CRL-1831 and for CSC-DLD1, respectively, were enriched. Next, we grouped these pathways into three functional categories: cancer transformation/metastasis, cell interaction, and stemness. ?-catenin (CTNNB1) was confirmed as a hub gene of all three functional categories. CONCLUSIONS:Our present findings suggest common pathways between reprogrammed human colon normal epithelium (iPSC-CRL-1831) and adenocarcinoma (CSC-DLD1) cells grown under 3D microenvironment. In addition, we demonstrated that pathways important for cancer transformation and tumor metastatic activity are altered both in normal and cancer stem-like cells during the transfer from 2D to 3D culture conditions. Thus, we indicate the potential of cell culture models enriched in normal and cancer stem-like cells for the identification of new therapeutic targets in cancer treatment.

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Histone acetylation regulates p21WAF1 expression in human colon cancer cell lines.

Publications

Histone acetylation regulates p21WAF1 expression in human colon cancer cell lines.

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