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Development and characterization of salivary adenoid cystic carcinoma cell line.


ABSTRACT: To develop in vitro adenoid cystic carcinoma cell line as a surrogate for functional studies.Cells obtained from a primary ACC of the base of tongue were cultivated in vitro and immortalized with h-TERT. Morphologic, cytogenetic and functional studies were performed.Tumor cells were verified by positive reactions to keratin and smooth muscle actin and phenotypic cellular and nuclear features. In-vitro cell growth and colony formation assay supported their tumor nature.We authenticated an ACC cell line with hybrid epithelial-myoepithelial feature as a resource for functional experimentation.

SUBMITTER: Li J 

PROVIDER: S-EPMC4573592 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature

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Development and characterization of salivary adenoid cystic carcinoma cell line.

Li Jie J   Perlaky Laszlo L   Rao Pulivarthi P   Weber Randal S RS   El-Naggar Adel K AK  

Oral oncology 20140730 10


<h4>Objective</h4>To develop in vitro adenoid cystic carcinoma cell line as a surrogate for functional studies.<h4>Materials and methods</h4>Cells obtained from a primary ACC of the base of tongue were cultivated in vitro and immortalized with h-TERT. Morphologic, cytogenetic and functional studies were performed.<h4>Results</h4>Tumor cells were verified by positive reactions to keratin and smooth muscle actin and phenotypic cellular and nuclear features. In-vitro cell growth and colony formatio  ...[more]

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