Chloramphenicol Selection of IS10 Transposition in the cat Promoter Region of Widely Used Cloning Vectors.
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ABSTRACT:
SUBMITTER: Gonzalez-Prieto C
PROVIDER: S-EPMC4574204 | biostudies-literature | 2015
REPOSITORIES: biostudies-literature
Publications
Chloramphenicol Selection of IS10 Transposition in the cat Promoter Region of Widely Used Cloning Vectors.
González-Prieto Coral C Agúndez Leticia L Llosa Matxalen M
PloS one 20150916 9
The widely used pSU8 family of cloning vectors is based on a p15A replicon and a chloramphenicol acetyltransferase (cat) gene conferring chloramphenicol resistance. We frequently observed an increase in the size of plasmids derived from these vectors. Analysis of the bigger molecular species shows that they have an IS10 copy inserted at a specific site between the promoter and the cat open reading frame. Promoter activity from both ends of IS10 has been reported, suggesting that the insertion ev ...[more]
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