Ontology highlight
ABSTRACT:
SUBMITTER: Dungrawala H
PROVIDER: S-EPMC4575883 | biostudies-literature | 2015 Sep
REPOSITORIES: biostudies-literature
Dungrawala Huzefa H Rose Kristie L KL Bhat Kamakoti P KP Mohni Kareem N KN Glick Gloria G GG Couch Frank B FB Cortez David D
Molecular cell 20150910 6
The ATR replication checkpoint ensures that stalled forks remain stable when replisome movement is impeded. Using an improved iPOND protocol combined with SILAC mass spectrometry, we characterized human replisome dynamics in response to fork stalling. Our data provide a quantitative picture of the replisome and replication stress response proteomes in 32 experimental conditions. Importantly, rather than stabilize the replisome, the checkpoint prevents two distinct types of fork collapse. Unsuper ...[more]