Expression of hepatitis C virus envelope protein 2 induces apoptosis in cultured mammalian cells.
Ontology highlight
ABSTRACT: Aim
To explore the role of hepatitis C virus (HCV) envelope protein 2 (E2) in the induction of apoptosis.Methods
A carboxyterminal truncated E2 (E2-661) was transiently expressed in several cultured mammalian cell lines or stably expressed in Chinese hamster ovary (CHO) cell line. Cell proliferation was assessed by 3H thymidine uptake. Apoptosis was examined by Hoechst 33258 staining, flow cytometry and DNA fragmentation analysis.Results
Reduced proliferation was readily observed in the E2-661 expressing cells. These cells manifested the typical features of apoptosis, including cell shrinkage, chromatin condensation and hypodiploid genomic DNA content. Similar apoptotic cell death was observed in an E2-661 stably expressing cell line.Conclusion
HCV E2 can induce apoptosis in cultured mammalian cells.
SUBMITTER: Zhu LX
PROVIDER: S-EPMC4576255 | biostudies-literature | 2004 Oct
REPOSITORIES: biostudies-literature
Publications
Expression of hepatitis C virus envelope protein 2 induces apoptosis in cultured mammalian cells.
Zhu Li-Xin LX Liu Jing J Xie You-Hua YH Kong Yu-Ying YY Ye Ye Y Wang Chun-Lin CL Li Guang-Di GD Wang Yuan Y
World journal of gastroenterology 20041001 20
<h4>Aim</h4>To explore the role of hepatitis C virus (HCV) envelope protein 2 (E2) in the induction of apoptosis.<h4>Methods</h4>A carboxyterminal truncated E2 (E2-661) was transiently expressed in several cultured mammalian cell lines or stably expressed in Chinese hamster ovary (CHO) cell line. Cell proliferation was assessed by 3H thymidine uptake. Apoptosis was examined by Hoechst 33258 staining, flow cytometry and DNA fragmentation analysis.<h4>Results</h4>Reduced proliferation was readily ...[more]
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