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Gene expression profile in liver of hB1F transgenic mice.


ABSTRACT: AIM:To analyze the tissue morphologic phenotype and liver gene expression profile of hB1F transgenic mice. METHODS:Transgene expression was analyzed with RT-PCR and Western blotting. For one of the transgenic mouse lines, tissue expression pattern of the transgene was also examined with immunochemical methods. Pathological analysis was used to examine the tissue morphologic phenotype of established transgenic mice. The liver gene expression profile of transgenic mice was analyzed with microchip, and some of the differentially expressed genes were verified with RT-PCR. RESULTS:The expressions of hB1F were shown in livers from 6 of 7 transgenic mouse lines. The overexpression of hB1F transgene did not cause pathological changes. Expressions of three genes were up-regulated, while down-regulation was observed for 25 genes. CONCLUSION:The overexpression of hB1F transgene may cause changes of gene expression profiles in the liver of transgenic mice.

SUBMITTER: Wang SL 

PROVIDER: S-EPMC4576262 | biostudies-literature | 2004 Oct

REPOSITORIES: biostudies-literature

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Gene expression profile in liver of hB1F transgenic mice.

Wang Shui-Liang SL   Yang Hua H   Xie You-Hua YH   Wang Yuan Y   Li Jian-Zhong JZ   Wang Long L   Wang Zhu-Gang ZG   Fu Ji-Liang JL  

World journal of gastroenterology 20041001 20


<h4>Aim</h4>To analyze the tissue morphologic phenotype and liver gene expression profile of hB1F transgenic mice.<h4>Methods</h4>Transgene expression was analyzed with RT-PCR and Western blotting. For one of the transgenic mouse lines, tissue expression pattern of the transgene was also examined with immunochemical methods. Pathological analysis was used to examine the tissue morphologic phenotype of established transgenic mice. The liver gene expression profile of transgenic mice was analyzed  ...[more]

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