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Non-Uniform Sampling and J-UNIO Automation for Efficient Protein NMR Structure Determination.

ABSTRACT: High-resolution structure determination of small proteins in solution is one of the big assets of NMR spectroscopy in structural biology. Improvements in the efficiency of NMR structure determination by advances in NMR experiments and automation of data handling therefore attracts continued interest. Here, non-uniform sampling (NUS) of 3D heteronuclear-resolved [(1)H,(1)H]-NOESY data yielded two- to three-fold savings of instrument time for structure determinations of soluble proteins. With the 152-residue protein NP_372339.1 from Staphylococcus aureus and the 71-residue protein NP_346341.1 from Streptococcus pneumonia we show that high-quality structures can be obtained with NUS NMR data, which are equally well amenable to robust automated analysis as the corresponding uniformly sampled data.

SUBMITTER: Didenko T

PROVIDER: S-EPMC4576834 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature

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Non-Uniform Sampling and J-UNIO Automation for Efficient Protein NMR Structure Determination.

Didenko Tatiana T Proudfoot Andrew A Dutta Samit Kumar SK Serrano Pedro P Wüthrich Kurt K

Chemistry (Weinheim an der Bergstrasse, Germany) 20150728 35

High-resolution structure determination of small proteins in solution is one of the big assets of NMR spectroscopy in structural biology. Improvements in the efficiency of NMR structure determination by advances in NMR experiments and automation of data handling therefore attracts continued interest. Here, non-uniform sampling (NUS) of 3D heteronuclear-resolved [(1)H,(1)H]-NOESY data yielded two- to three-fold savings of instrument time for structure determinations of soluble proteins. With the ...[more]

PMID: 26227870

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