Dataset Information

Expression and Function of Hypoxia Inducible Factor-1? and Vascular Endothelial Growth Factor in Pulp Tissue of Teeth under Orthodontic Movement.

ABSTRACT: Orthodontic force may lead to cell damage, circulatory disturbances, and vascular changes of the dental pulp, which make a hypoxic environment in pulp. In order to maintain the homeostasis of dental pulp, hypoxia will inevitably induce the defensive reaction. However, this is a complex process and is regulated by numerous factors. In this study, we established an experimental animal model of orthodontic tooth movement to investigate the effects of mechanical force on the expression of VEGF and HIF-1? in dental pulp. Histological analysis of dental pulp and expressions of HIF-1? and VEGF proteins in dental pulp were examined. The results showed that inflammation and vascular changes happened in dental pulp tissue in different periods. Additionally, there were significant changes in the expression of HIF-1? and VEGF proteins under orthodontic force. After application of mechanical load, expression of HIF-1? and VEGF was markedly positive in 1, 3, 7?d, and 2?w groups, and then it weakened in 4?w group. These findings suggested that the expression of HIF-1? and VEGF was enhanced by mechanical force. HIF-1? and VEGF may play an important role in retaining the homeostasis of dental pulp during orthodontic tooth movement.

SUBMITTER: Wei F

PROVIDER: S-EPMC4579319 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Expression and Function of Hypoxia Inducible Factor-1α and Vascular Endothelial Growth Factor in Pulp Tissue of Teeth under Orthodontic Movement.

Wei Fulan F Yang Shuangyan S Xu Hui H Guo Qingyuan Q Li Qi Q Hu Lihua L Liu Dongxu D Wang Chunling C

Mediators of inflammation 20150909

Orthodontic force may lead to cell damage, circulatory disturbances, and vascular changes of the dental pulp, which make a hypoxic environment in pulp. In order to maintain the homeostasis of dental pulp, hypoxia will inevitably induce the defensive reaction. However, this is a complex process and is regulated by numerous factors. In this study, we established an experimental animal model of orthodontic tooth movement to investigate the effects of mechanical force on the expression of VEGF and H ...[more]

PMID: 26441483

Similar Datasets

Project description:BackgroundDiffering levels of tendon retraction are found in full-thickness rotator cuff tears. The pathophysiology of tendon degeneration and retraction is unclear. Neoangiogenesis in tendon parenchyma indicates degeneration. Hypoxia inducible factor 1α (HIF) and vascular endothelial growth factor (VEGF) are important inducers of neoangiogenesis. Rotator cuff tendons rupture leads to fatty muscle infiltration (FI) and muscle atrophy (MA). The aim of this study is to clarify the relationship between HIF and VEGF expression, neoangiogenesis, FI, and MA in tendon retraction found in full-thickness rotator cuff tears.MethodsRotator cuff tendon samples of 33 patients with full-thickness medium-sized rotator cuff tears were harvested during reconstructive surgery. The samples were dehydrated and paraffin embedded. For immunohistological determination of VEGF and HIF expression, sample slices were strained with VEGF and HIF antibody dilution. Vessel density and vessel size were determined after Masson-Goldner staining of sample slices. The extent of tendon retraction was determined intraoperatively according to Patte's classification. Patients were assigned to 4 categories based upon Patte tendon retraction grade, including one control group. FI and MA were measured on standardized preoperative shoulder MRI.ResultsHIF and VEGF expression, FI, and MA were significantly higher in torn cuff samples compared with healthy tissue (p < 0.05). HIF and VEGF expression, and vessel density significantly increased with extent of tendon retraction (p < 0.05). A correlation between HIF/VEGF expression and FI and MA could be found (p < 0.05). There was no significant correlation between HIF/VEGF expression and neovascularity (p > 0.05)ConclusionTendon retraction in full-thickness medium-sized rotator cuff tears is characterized by neovascularity, increased VEGF/HIF expression, FI, and MA. VEGF expression and neovascularity may be effective monitoring tools to assess tendon degeneration.

Dataset Information

Expression and Function of Hypoxia Inducible Factor-1? and Vascular Endothelial Growth Factor in Pulp Tissue of Teeth under Orthodontic Movement.

Publications

Expression and Function of Hypoxia Inducible Factor-1α and Vascular Endothelial Growth Factor in Pulp Tissue of Teeth under Orthodontic Movement.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets