Unknown

Dataset Information

0

Amino acids 89-96 of Salmonella typhimurium flagellin represent the major domain responsible for TLR5-independent adjuvanticity in the humoral immune response.


ABSTRACT: Toll-like receptor 5 (TLR5) signaling in response to flagellin is dispensable for inducing humoral immunity, but alterations of aa 89-96, the TLR5 binding site, significantly reduced the adjuvanticity of flagellin. These observations indicate that the underlying mechanism remains incompletely understood. Here, we found that the native form of Salmonella typhimurium aa 89-96-mutant flagellin extracted from flagella retains some TLR5 recognition activity, indicating that aa 89-96 is the primary, but not the only site that imparts TLR5 activity. Additionally, this mutation impaired the production of IL-1? and IL-18. Using TLR5KO mice, we found that aa 89-96 is critical for the humoral adjuvant effect, but this effect was independent of TLR5 activation triggered by this region of flagellin. In summary, our findings suggest that aa 89-96 of flagellin is not only the crucial site responsible for TLR5 recognition, but is also important for humoral immune adjuvanticity through a TLR5-independent pathway.

SUBMITTER: Zhang L 

PROVIDER: S-EPMC4579647 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature

altmetric image

Publications

Amino acids 89-96 of Salmonella typhimurium flagellin represent the major domain responsible for TLR5-independent adjuvanticity in the humoral immune response.

Zhang Lei L   Pan Zhiming Z   Kang Xilong X   Yang Yun Y   Kang Heekap H   Zhang Na N   Rosati James M JM   Jiao Xinan X  

Cellular & molecular immunology 20140908 5


Toll-like receptor 5 (TLR5) signaling in response to flagellin is dispensable for inducing humoral immunity, but alterations of aa 89-96, the TLR5 binding site, significantly reduced the adjuvanticity of flagellin. These observations indicate that the underlying mechanism remains incompletely understood. Here, we found that the native form of Salmonella typhimurium aa 89-96-mutant flagellin extracted from flagella retains some TLR5 recognition activity, indicating that aa 89-96 is the primary, b  ...[more]

Similar Datasets

| S-EPMC3498291 | biostudies-literature
| S-EPMC3406927 | biostudies-literature
| S-EPMC8546927 | biostudies-literature
| S-EPMC5732158 | biostudies-literature
| S-EPMC4287482 | biostudies-literature
| S-EPMC3665710 | biostudies-literature
| S-EPMC1196272 | biostudies-literature
| S-EPMC3294599 | biostudies-literature
| S-EPMC5247705 | biostudies-literature
| S-EPMC6420342 | biostudies-literature