Flexibility of NS5 Methyltransferase-Polymerase Linker Region Is Essential for Dengue Virus Replication.
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ABSTRACT:
SUBMITTER: Zhao Y
PROVIDER: S-EPMC4580200 | biostudies-literature | 2015 Oct
REPOSITORIES: biostudies-literature
Publications
Flexibility of NS5 Methyltransferase-Polymerase Linker Region Is Essential for Dengue Virus Replication.
Zhao Yongqian Y Soh Tingjin Sherryl TS Chan Kitti Wing Ki KW Fung Sarah Suet Yin SS Swaminathan Kunchithapadam K Lim Siew Pheng SP Shi Pei-Yong PY Huber Thomas T Lescar Julien J Luo Dahai D Vasudevan Subhash G SG
Journal of virology 20150812 20
We examined the function of the conserved Val/Ile residue within the dengue virus NS5 interdomain linker (residues 263 to 272) by site-directed mutagenesis. Gly substitution or Gly/Pro insertion after the conserved residue increased the linker flexibility and created slightly attenuated viruses. In contrast, Pro substitution abolished virus replication by imposing rigidity in the linker and restricting NS5's conformational plasticity. Our biochemical and reverse genetics experiments demonstrate ...[more]
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