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Mutations in the histamine N-methyltransferase gene, HNMT, are associated with nonsyndromic autosomal recessive intellectual disability.

ABSTRACT: Histamine (HA) acts as a neurotransmitter in the brain, which participates in the regulation of many biological processes including inflammation, gastric acid secretion and neuromodulation. The enzyme histamine N-methyltransferase (HNMT) inactivates HA by transferring a methyl group from S-adenosyl-l-methionine to HA, and is the only well-known pathway for termination of neurotransmission actions of HA in mammalian central nervous system. We performed autozygosity mapping followed by targeted exome sequencing and identified two homozygous HNMT alterations, p.Gly60Asp and p.Leu208Pro, in patients affected with nonsyndromic autosomal recessive intellectual disability from two unrelated consanguineous families of Turkish and Kurdish ancestry, respectively. We verified the complete absence of a functional HNMT in patients using in vitro toxicology assay. Using mutant and wild-type DNA constructs as well as in silico protein modeling, we confirmed that p.Gly60Asp disrupts the enzymatic activity of the protein, and that p.Leu208Pro results in reduced protein stability, resulting in decreased HA inactivation. Our results highlight the importance of inclusion of HNMT for genetic testing of individuals presenting with intellectual disability.

SUBMITTER: Heidari A 

PROVIDER: S-EPMC4581600 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature

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Mutations in the histamine N-methyltransferase gene, HNMT, are associated with nonsyndromic autosomal recessive intellectual disability.

Heidari Abolfazl A   Tongsook Chanakan C   Najafipour Reza R   Musante Luciana L   Vasli Nasim N   Garshasbi Masoud M   Hu Hao H   Mittal Kirti K   McNaughton Amy J M AJ   Sritharan Kumudesh K   Hudson Melissa M   Stehr Henning H   Talebi Saeid S   Moradi Mohammad M   Darvish Hossein H   Arshad Rafiq Muhammad M   Mozhdehipanah Hossein H   Rashidinejad Ali A   Samiei Shahram S   Ghadami Mohsen M   Windpassinger Christian C   Gillessen-Kaesbach Gabriele G   Tzschach Andreas A   Ahmed Iltaf I   Mikhailov Anna A   Stavropoulos D James DJ   Carter Melissa T MT   Keshavarz Soraya S   Ayub Muhammad M   Najmabadi Hossein H   Liu Xudong X   Ropers Hans Hilger HH   Macheroux Peter P   Vincent John B JB  

Human molecular genetics 20150723 20

Histamine (HA) acts as a neurotransmitter in the brain, which participates in the regulation of many biological processes including inflammation, gastric acid secretion and neuromodulation. The enzyme histamine N-methyltransferase (HNMT) inactivates HA by transferring a methyl group from S-adenosyl-l-methionine to HA, and is the only well-known pathway for termination of neurotransmission actions of HA in mammalian central nervous system. We performed autozygosity mapping followed by targeted ex  ...[more]

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