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Comparison of blood plasma sample preparation methods for combined LC-MS lipidomics and metabolomics.

ABSTRACT: The goal of this research was to find the most comprehensive lipid extraction of blood plasma, while also providing adequate aqueous preparation for metabolite analysis. Comparisons have been made previously of the Folch, Bligh-Dyer, and Matyash lipid extractions; furthermore, this paper provides an additional comparison of a phospholipid removal plate for analysis. This plate was used for lipid extraction rather than its intended use in lipid removal for polar analysis, and it proves to be robust for targeted lipid analysis. Folch and Matyash provided reproducible recovery over a range of lipid classes, however the Matyash aqueous layer compared well to a typical methanol preparation for polar metabolite analysis. Thus, the Matyash method is the best choice for an untargeted biphasic extraction for metabolomics and lipidomics in blood plasma.

SUBMITTER: Patterson RE

PROVIDER: S-EPMC4582001 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature

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Comparison of blood plasma sample preparation methods for combined LC-MS lipidomics and metabolomics.

Patterson Rainey E RE Ducrocq Antoine J AJ McDougall Danielle J DJ Garrett Timothy J TJ Yost Richard A RA

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences 20150828

The goal of this research was to find the most comprehensive lipid extraction of blood plasma, while also providing adequate aqueous preparation for metabolite analysis. Comparisons have been made previously of the Folch, Bligh-Dyer, and Matyash lipid extractions; furthermore, this paper provides an additional comparison of a phospholipid removal plate for analysis. This plate was used for lipid extraction rather than its intended use in lipid removal for polar analysis, and it proves to be robu ...[more]

PMID: 26343017

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