Ontology highlight
ABSTRACT:
SUBMITTER: Suksombat S
PROVIDER: S-EPMC4582245 | biostudies-literature | 2015 Aug
REPOSITORIES: biostudies-literature
Suksombat Sukrit S Khafizov Rustem R Kozlov Alexander G AG Lohman Timothy M TM Chemla Yann R YR
eLife 20150825
Escherichia coli single-stranded (ss)DNA binding (SSB) protein mediates genome maintenance processes by regulating access to ssDNA. This homotetrameric protein wraps ssDNA in multiple distinct binding modes that may be used selectively in different DNA processes, and whose detailed wrapping topologies remain speculative. Here, we used single-molecule force and fluorescence spectroscopy to investigate E. coli SSB binding to ssDNA. Stretching a single ssDNA-SSB complex reveals discrete states that ...[more]