Genome-Wide Assessment of Outer Membrane Vesicle Production in Escherichia coli.
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ABSTRACT:
SUBMITTER: Kulp AJ
PROVIDER: S-EPMC4583269 | biostudies-literature | 2015
REPOSITORIES: biostudies-literature
Publications
Genome-Wide Assessment of Outer Membrane Vesicle Production in Escherichia coli.
Kulp Adam J AJ Sun Bo B Ai Teresa T Manning Andrew J AJ Orench-Rivera Nichole N Schmid Amy K AK Kuehn Meta J MJ
PloS one 20150925 9
The production of outer membrane vesicles by Gram-negative bacteria has been well documented; however, the mechanism behind the biogenesis of these vesicles remains unclear. Here a high-throughput experimental method and systems-scale analysis was conducted to determine vesiculation values for the whole genome knockout library of Escherichia coli mutant strains (Keio collection). The resultant dataset quantitatively recapitulates previously observed phenotypes and implicates nearly 150 new genes ...[more]
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