Ontology highlight
ABSTRACT:
SUBMITTER: Stelzer Y
PROVIDER: S-EPMC4583717 | biostudies-literature | 2015 Sep
REPOSITORIES: biostudies-literature
Stelzer Yonatan Y Shivalila Chikdu Shakti CS Soldner Frank F Markoulaki Styliani S Jaenisch Rudolf R
Cell 20150901 1
Mammalian DNA methylation plays an essential role in development. To date, only snapshots of different mouse and human cell types have been generated, providing a static view on DNA methylation. To enable monitoring of methylation status as it changes over time, we establish a reporter of genomic methylation (RGM) that relies on a minimal imprinted gene promoter driving a fluorescent protein. We show that insertion of RGM proximal to promoter-associated CpG islands reports the gain or loss of DN ...[more]