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Isolation and molecular characterization of Orf virus from natural outbreaks in goats of Assam.

ABSTRACT: Outbreaks of contagious ecthyma (caused by a Parapox virus) in goats were investigated in 6 districts of Assam, a north eastern state of India. Diagnosis of the disease was carried out employing both standard virological as well as molecular methods. Four representative isolates from different places were selected for phylogenetic analysis. The major envelop protein (B2L) of Orf virus was targeted for molecular analysis. The sequencing and phylogenetic analysis of the selected sequences at nucleotide level revealed that the Orf virus isolates were closely related to each other (97.6-100 %) and showed highest similarity to the Orf virus isolate 82/04 (98.4 %), reported from Shahjahanpur, India. The data will provide an insight in transmission of the virus from northern to North eastern part of the country.

SUBMITTER: Bora M

PROVIDER: S-EPMC4585050 | biostudies-literature | 2015 Jun

REPOSITORIES: biostudies-literature

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Isolation and molecular characterization of Orf virus from natural outbreaks in goats of Assam.

Bora Mousumi M Bora Durlav Prasad DP Barman Nagendra Nath NN Borah Biswajyoti B Bora Padma Lochan PL Talukdar Archana A Tamuly Shantanu S

Virusdisease 20150623 1-2

Outbreaks of contagious ecthyma (caused by a Parapox virus) in goats were investigated in 6 districts of Assam, a north eastern state of India. Diagnosis of the disease was carried out employing both standard virological as well as molecular methods. Four representative isolates from different places were selected for phylogenetic analysis. The major envelop protein (B2L) of Orf virus was targeted for molecular analysis. The sequencing and phylogenetic analysis of the selected sequences at nucle ...[more]

PMID: 26436126

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Molecular characterization of Orf virus in goats in Gabon, Central Africa.

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| S-EPMC4866431 | biostudies-literature

Molecular characterization of orf virus from sheep and goats in Ethiopia, 2008-2013.

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Molecular characterization and phylogenetic analysis of orf virus isolated from goats in Sokoto metropolis, Nigeria.

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Phylogenetic analysis and characterization of Korean orf virus from dairy goats: case report.

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Phylogenetic Analysis of ORF Viruses From Five Contagious Ecthyma Outbreaks in Argentinian Goats.

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Human infection with ORF virus from goats in China, 2012.

Project description:Orf virus, which belongs to the Parapoxvirus genus, induces a zoonotic infectious disease characterized by acute, highly vascularized cutaneous pustular lesions in sheep and goats. A number of Orf outbreaks have been reported in sheep and goats in recent years, but no reports have described an Orf virus strain from humans in China. In this study, we diagnosed Orf virus infection in two people, a mother and son, in the Gansu province of China. The human Orf virus was isolated and its phylogenetic characterization was analyzed based on a complete B2L gene. The results are useful for developing prospective programs to control Orf virus infections in both goats and humans.

| S-EPMC4026105 | biostudies-literature

Heterogeneity among orf virus isolates from goats in Fujian Province, Southern China.

Project description:Orf virus is a parapoxvirus that causes recurring contagious ecthyma or orf disease in goat, sheep and other wild and domestic ruminants. Infected animals show signs of pustular lesions on the mouth and muzzle and develop scabs over the lesions. Although the infection is usually cleared within 1-2 months, delayed growth and associated secondary infections could still impact the herds. Orf virus can also infect humans, causing lesions similar to the animals in pathological histology. Prior infection of orf virus apparently offers little protective immunity against future infections. Several gene products of orf virus have been identified as responsible for immunomodulatory functions. In our recent study of orf virus isolates from an area along the Minjiang River in northern Fujian Province, we found a high heterogeneity among isolates from 10 farms within a 120-kilometer distance. Only two isolates from locations within 1 km to each other had same viral genes. There is no correlation between the geographical distance between the corresponding collection sites and the phylogenetic distance in ORFV011 or ORV059 genes for any two isolates. This finding suggests that there are diverse populations of orf virus present in the environment. This may in part contribute to the phenomenon of recurring outbreaks and heighten the need for better surveillance.

| S-EPMC3797069 | biostudies-literature

Molecular characterization of Orf virus isolates from Kodai hills, Tamil Nadu, India.

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Isolation and Characterization of Monoclonal Antibodies Against a Virion Core Protein of Orf Virus Strain NA1/11 As Potential Diagnostic Tool for Orf Viruses.

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