Ontology highlight
ABSTRACT:
SUBMITTER: Larion M
PROVIDER: S-EPMC4587531 | biostudies-literature | 2015 Jul
REPOSITORIES: biostudies-literature
Angewandte Chemie (International ed. in English) 20150526 28
The hallmark of glucokinase (GCK), which catalyzes the phosphorylation of glucose during glycolysis, is its kinetic cooperativity, whose understanding at atomic detail has remained open since its discovery over 40 years ago. Herein, by using kinetic CPMG NMR spectroscopic data for 17 isoleucine side chains distributed over all parts of GCK, we show that the origin of kinetic cooperativity is rooted in intramolecular protein dynamics. Residues of glucose-free GCK located in the small domain displ ...[more]