Ontology highlight
ABSTRACT:
SUBMITTER: Aits S
PROVIDER: S-EPMC4590643 | biostudies-literature | 2015
REPOSITORIES: biostudies-literature
Aits Sonja S Kricker Jennifer J Liu Bin B Ellegaard Anne-Marie AM Hämälistö Saara S Tvingsholm Siri S Corcelle-Termeau Elisabeth E Høgh Søren S Farkas Thomas T Holm Jonassen Anna A Gromova Irina I Mortensen Monika M Jäättelä Marja M
Autophagy 20150101 8
Lysosomal membrane permeabilization (LMP) contributes to tissue involution, degenerative diseases, and cancer therapy. Its investigation has, however, been hindered by the lack of sensitive methods. Here, we characterize and validate the detection of galectin puncta at leaky lysosomes as a highly sensitive and easily manageable assay for LMP. LGALS1/galectin-1 and LGALS3/galectin-3 are best suited for this purpose due to their widespread expression, rapid translocation to leaky lysosomes and ava ...[more]