Ontology highlight
ABSTRACT:
SUBMITTER: Liu J
PROVIDER: S-EPMC4591219 | biostudies-literature | 2015 Sep
REPOSITORIES: biostudies-literature
Liu Jinqiang J Yu Clinton C Hu Xichan X Kim Jin-Kwang JK Bierma Jan C JC Jun Hyun-Ik HI Rychnovsky Scott D SD Huang Lan L Qiao Feng F
Cell reports 20150910 12
Shelterin, a six-member complex, protects telomeres from nucleolytic attack and regulates their elongation by telomerase. Here, we have developed a strategy, called MICro-MS (Mapping Interfaces via Crosslinking-Mass Spectrometry), that combines crosslinking-mass spectrometry and phylogenetic analysis to identify contact sites within the complex. This strategy allowed identification of separation-of-function mutants of fission yeast Ccq1, Poz1, and Pot1 that selectively disrupt their respective i ...[more]