Arterial Expression of the Calcium-Sensing Receptor Is Maintained by Physiological Pulsation and Protects against Calcification.
Ontology highlight
ABSTRACT: Unlabelled
Vascular calcification (VC) is common in chronic kidney disease (CKD) and contributes to cardiovascular mortality. The calcium-sensing receptor (CaSR) is present in human artery, senses extracellular calcium and may directly modulate VC.Objective
to investigate the association between arterial cyclic strain, CaSR expression and VC.Methods and results
human aortic smooth muscle cells (HAoSMC) were cultured under static or strained conditions, with exposure to CaSR agonists, the calcimimetic R568, and after CaSR silencing and over-expression. High extracellular calcium reduced CaSR expression and promoted osteochondrogenic transformation and calcium deposition. This was partially prevented by cyclic strain and exposure to R568. CaSR silencing enhanced calcification and osteochondrogenic transformation, whereas CaSR over-expression attenuated this procalcific response, demonstrating a central role for the CaSR in the response to cyclic strain and regulation of VC. In arterial explants from CKD patients (n = 11) and controls (n = 9), exposure to R568 did not significantly alter calcium deposition, osteochondrogenic markers or total artery calcium content.Conclusions
physiological mechanical strain is important for arterial homeostasis and may protect arteries from VC. The beneficial effects of cyclic strain may be mediated via the CaSR.
SUBMITTER: Molostvov G
PROVIDER: S-EPMC4593585 | biostudies-literature | 2015
REPOSITORIES: biostudies-literature
Publications
Arterial Expression of the Calcium-Sensing Receptor Is Maintained by Physiological Pulsation and Protects against Calcification.
Molostvov Guerman G Hiemstra Thomas F TF Fletcher Simon S Bland Rosemary R Zehnder Daniel D
PloS one 20151005 10
<h4>Unlabelled</h4>Vascular calcification (VC) is common in chronic kidney disease (CKD) and contributes to cardiovascular mortality. The calcium-sensing receptor (CaSR) is present in human artery, senses extracellular calcium and may directly modulate VC.<h4>Objective</h4>to investigate the association between arterial cyclic strain, CaSR expression and VC.<h4>Methods and results</h4>human aortic smooth muscle cells (HAoSMC) were cultured under static or strained conditions, with exposure to Ca ...[more]
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