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Cofactor-induced reversible folding of Flavodoxin-4 from Lactobacillus acidophilus.


ABSTRACT: Flavodoxins in combination with the flavin mononucleotide (FMN) cofactor play important roles for electron transport in prokaryotes. Here, novel insights into the FMN-binding mechanism to flavodoxins-4 were obtained from the NMR structures of the apo-protein from Lactobacillus acidophilus (YP_193882.1) and comparison of its complex with FMN. Extensive reversible conformational changes were observed upon FMN binding and release. The NMR structure of the FMN complex is in agreement with the crystal structure (PDB ID: 3EDO) and exhibits the characteristic flavodoxin fold, with a central five-stranded parallel ?-sheet and five ?-helices forming an ?/?-sandwich architecture. The structure differs from other flavoproteins in that helix ?2 is oriented perpendicular to the ?-sheet and covers the FMN-binding site. This helix reversibly unfolds upon removal of the FMN ligand, which represents a unique structural rearrangement among flavodoxins.

SUBMITTER: Dutta SK 

PROVIDER: S-EPMC4594659 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature

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Cofactor-induced reversible folding of Flavodoxin-4 from Lactobacillus acidophilus.

Dutta Samit Kumar SK   Serrano Pedro P   Geralt Michael M   Axelrod Herbert L HL   Xu Qingping Q   Lesley Scott A SA   Godzik Adam A   Deacon Ashley M AM   Elsliger Marc-André MA   Wilson Ian A IA   Wüthrich Kurt K  

Protein science : a publication of the Protein Society 20150730 10


Flavodoxins in combination with the flavin mononucleotide (FMN) cofactor play important roles for electron transport in prokaryotes. Here, novel insights into the FMN-binding mechanism to flavodoxins-4 were obtained from the NMR structures of the apo-protein from Lactobacillus acidophilus (YP_193882.1) and comparison of its complex with FMN. Extensive reversible conformational changes were observed upon FMN binding and release. The NMR structure of the FMN complex is in agreement with the crysta  ...[more]

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