Unknown

Dataset Information

0

BcRISP1, isolated from non-heading Chinese cabbage, decreases the seed set of transgenic Arabidopsis.


ABSTRACT: Mitochondria are the energy sources of plant cells and are involved in regulating cell development. Ubiquinol-cytochrome c reductase iron-sulfur protein, which is necessary for mitochondrial respiration, is a subunit of mitochondrial electron transport chain multimeric enzyme complexes. To better understand the biological function of the ubiquinol-cytochrome c reductase iron-sulfur protein, the full-length cDNA of BcRISP1 was cloned; it was found to contain 810 base pairs and encode 269 amino acids. Unusually, high expression of the BcRISP1 gene in the archesporial cell stages was determined by quantitative reverse transcription-polymerase chain reaction (qRT-PCR) analysis of cytoplasmic male sterile lines and maintainer lines. The seed set was affected by the overexpression of BcRISP1, and shorter siliques with lower seed sets were observed in 35S::BcRISP1 Arabidopsis plants. These characteristics may have resulted from the reduced formation of pollen and impaired pollen tube growth. qRT-PCR results revealed that in 35S::BcRISP1 plants, the expression levels of the mitochondrial respiratory chain-related genes, COX10 and RIP1, were enhanced, whereas the expression levels of QCR7 and SDH2-1 were reduced. This result implies that overexpression of BcRISP1 in transgenic Arabidopsis plants may disrupt the mitochondrial electron transport chain by affecting the expression of mitochondrial respiratory chain-related genes and therefore, reducing the seed set.

SUBMITTER: Liu T 

PROVIDER: S-EPMC4596333 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature

altmetric image

Publications

BcRISP1, isolated from non-heading Chinese cabbage, decreases the seed set of transgenic Arabidopsis.

Liu Tongkun T   Qian Yu Y   Duan Weike W   Ren Jun J   Hou Xilin X   Li Ying Y  

Horticulture research 20141210


Mitochondria are the energy sources of plant cells and are involved in regulating cell development. Ubiquinol-cytochrome c reductase iron-sulfur protein, which is necessary for mitochondrial respiration, is a subunit of mitochondrial electron transport chain multimeric enzyme complexes. To better understand the biological function of the ubiquinol-cytochrome c reductase iron-sulfur protein, the full-length cDNA of BcRISP1 was cloned; it was found to contain 810 base pairs and encode 269 amino ac  ...[more]

Similar Datasets

2014-06-14 | GSE38722 | GEO
2014-06-14 | E-GEOD-38722 | biostudies-arrayexpress
| S-EPMC7999546 | biostudies-literature
| S-EPMC6379458 | biostudies-literature
| S-EPMC7392980 | biostudies-literature
| S-EPMC3998049 | biostudies-literature
| PRJNA475006 | ENA
| S-EPMC8334874 | biostudies-literature
| S-EPMC6335391 | biostudies-literature
| S-EPMC4901065 | biostudies-literature