Ontology highlight
ABSTRACT:
SUBMITTER: Berlin S
PROVIDER: S-EPMC4597790 | biostudies-literature | 2015 Sep
REPOSITORIES: biostudies-literature
Berlin Shai S Carroll Elizabeth C EC Newman Zachary L ZL Okada Hitomi O HO Quinn Carson M CM Kallman Benjamin B Rockwell Nathan C NC Martin Shelley S SS Lagarias J Clark JC Isacoff Ehud Y EY
Nature methods 20150713 9
Circuit mapping requires knowledge of both structural and functional connectivity between cells. Although optical tools have been made to assess either the morphology and projections of neurons or their activity and functional connections, few probes integrate this information. We have generated a family of photoactivatable genetically encoded Ca(2+) indicators that combines attributes of high-contrast photolabeling with high-sensitivity Ca(2+) detection in a single-color protein sensor. We demo ...[more]