Ontology highlight
ABSTRACT:
SUBMITTER: Burke MC
PROVIDER: S-EPMC4599693 | biostudies-literature | 2015 Aug
REPOSITORIES: biostudies-literature
Burke Meghan C MC Wang Yan Y Lee Amanda E AE Dixon Emma Kimm EK Castaneda Carlos A CA Fushman David D Fenselau Catherine C
Analytical chemistry 20150728 16
Unexpected tryptic cleavage has been characterized at modified K48 residues in polyubiquitins. In particular, the tryptic products of all seven of the lysine-linked dimers of ubiquitin and of three trimers-linear Ub-(48)Ub-(48)Ub, linear Ub-(63)Ub-(63)Ub, and the branched trimer [Ub]2-(6,48)Ub-have been analyzed. In addition to the peptide products expected under commonly used tryptic conditions, we observe that peptides are formed with an unexpected ε-glycinylglycinyl-Lys carboxyl terminus when ...[more]