Ontology highlight
ABSTRACT:
SUBMITTER: Takahashi N
PROVIDER: S-EPMC4600761 | biostudies-literature | 2015 Oct
REPOSITORIES: biostudies-literature
Takahashi Noriko N Sawada Wakako W Noguchi Jun J Watanabe Satoshi S Ucar Hasan H Hayashi-Takagi Akiko A Yagishita Sho S Ohno Mitsuyo M Tokumaru Hiroshi H Kasai Haruo H
Nature communications 20151006
It remains unclear how readiness for Ca(2+)-dependent exocytosis depends on varying degrees of SNARE complex assembly. Here we directly investigate the SNARE assembly using two-photon fluorescence lifetime imaging (FLIM) of Förster resonance energy transfer (FRET) between three pairs of neuronal SNAREs in presynaptic boutons and pancreatic β cells in the islets of Langerhans. These FRET probes functionally rescue their endogenous counterparts, supporting ultrafast exocytosis. We show that trans- ...[more]