Crystallization and preliminary X-ray diffraction studies of frutalin, an ?-D-galactose-specific lectin from Artocarpus incisa seeds.

ABSTRACT: Frutalin is an ?-D-galactose-specific carbohydrate-binding glycoprotein with antitumour properties and is a powerful tool for tumour biomarker discovery. The crystallization and preliminary X-ray diffraction analysis of this lectin, which was isolated from Artocarpus incisa seeds, are reported here. Frutalin was purified and submitted to mass-spectrometric analysis. Diverse masses at approximately 16?kDa were observed in the deconvoluted spectra, which support the presence of isoforms. The best frutalin crystals were grown within a week in 0.1?M citric acid pH 3.5 which contained 25% PEG 3350 as a precipitant at 293?K, and diffracted to a maximum resolution of 1.81?Å. The monoclinic crystals belonged to space group I2, with unit-cell parameters a = 76.17, b = 74.56, c = 118.98?Å, ? = 96.56°. A molecular-replacement solution was obtained which indicated the presence of four monomers per asymmetric unit. Crystallographic refinement of the structure is in progress.

SUBMITTER: Monteiro-Moreira AC 

PROVIDER: S-EPMC4601592 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature