Ontology highlight
ABSTRACT:
SUBMITTER: Marino F
PROVIDER: S-EPMC4603548 | biostudies-literature | 2015 Sep
REPOSITORIES: biostudies-literature
Marino Fabio F Bern Marshall M Mommen Geert P M GPM Leney Aneika C AC van Gaans-van den Brink Jacqueline A M JAM Bonvin Alexandre M J J AMJJ Becker Christopher C van Els Cécile A C M CACM Heck Albert J R AJR
Journal of the American Chemical Society 20150819 34
We report unexpected mass spectrometric observations of glycosylated human leukocyte antigen (HLA) class I-bound peptides. Complemented by molecular modeling, in vitro enzymatic assays, and oxonium ion patterns, we propose that the observed O-linked glycans carrying up to five monosaccharides are extended O-GlcNAc's rather than GalNAc-initiated O-glycans. A cytosolic O-GlcNAc modification is normally terminal and does not extend to produce a polysaccharide, but O-GlcNAc on an HLA peptide present ...[more]