Characterization of fusion genes and the significantly expressed fusion isoforms in breast cancer by hybrid sequencing.
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ABSTRACT:
SUBMITTER: Weirather JL
PROVIDER: S-EPMC4605286 | biostudies-literature | 2015 Oct
REPOSITORIES: biostudies-literature
Publications
Characterization of fusion genes and the significantly expressed fusion isoforms in breast cancer by hybrid sequencing.
Weirather Jason L JL Afshar Pegah Tootoonchi PT Clark Tyson A TA Tseng Elizabeth E Powers Linda S LS Underwood Jason G JG Zabner Joseph J Korlach Jonas J Wong Wing Hung WH Au Kin Fai KF
Nucleic acids research 20150603 18
We developed an innovative hybrid sequencing approach, IDP-fusion, to detect fusion genes, determine fusion sites and identify and quantify fusion isoforms. IDP-fusion is the first method to study gene fusion events by integrating Third Generation Sequencing long reads and Second Generation Sequencing short reads. We applied IDP-fusion to PacBio data and Illumina data from the MCF-7 breast cancer cells. Compared with the existing tools, IDP-fusion detects fusion genes at higher precision and a v ...[more]
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