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Using AIE Luminogen for Long-term and Low-background Three-Photon Microscopic Functional Bioimaging.

ABSTRACT: Fluorescent probes are one of the most popularly used bioimaging markers to monitor metabolic processes of living cells. However, long-term light excitation always leads to photobleaching of fluorescent probes, unavoidable autofluorescence as well as photodamage of cells. To overcome these limitations, we synthesized a type of photostable luminogen named TPE-TPP with an aggregation induced emission (AIE) characteristic, and achieved its three-photon imaging with femtosecond laser excitation of 1020?nm. By using TPE-TPP as fluorescent probes, three-photon microscopy under 1020?nm excitation showed little photo-damage, as well as low autofluorescence to HeLa cells. Due to the AIE effect, the TPE-TPP nanoaggregates uptaken by cells were resistant to photobleaching under three-photon excitation for an extended period of time. Furthermore, we demonstrated that for the present TPE-TPP AIE the three-photon microscopy (with 1020?nm excitation) had a better signal to noise ratio than the two-photon microscopy (with 810?nm excitation) in tissue imaging.

SUBMITTER: Zhu Z

PROVIDER: S-EPMC4606727 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature

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Using AIE Luminogen for Long-term and Low-background Three-Photon Microscopic Functional Bioimaging.

Zhu Zhenfeng Z Leung Chris W T CW Zhao Xinyuan X Wang Yalun Y Qian Jun J Tang Ben Zhong BZ He Sailing S

Scientific reports 20151015

Fluorescent probes are one of the most popularly used bioimaging markers to monitor metabolic processes of living cells. However, long-term light excitation always leads to photobleaching of fluorescent probes, unavoidable autofluorescence as well as photodamage of cells. To overcome these limitations, we synthesized a type of photostable luminogen named TPE-TPP with an aggregation induced emission (AIE) characteristic, and achieved its three-photon imaging with femtosecond laser excitation of 1 ...[more]

PMID: 26470006

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Dataset Information

Using AIE Luminogen for Long-term and Low-background Three-Photon Microscopic Functional Bioimaging.

Publications

Using AIE Luminogen for Long-term and Low-background Three-Photon Microscopic Functional Bioimaging.

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