MicroRNA profiles reveal female allotetraploid hybrid fertility.

ABSTRACT:

Background

The bisexual fertile tetraploid fish is important in biological evolution. Tetraploid fish fertility is the key factor for stable inheritance. Therefore, elucidating tetraploid fish fertility at the molecular level is essential. MicroRNAs regulate gene expression and are involved in many aspects of gonad development.

Methods

Total RNA was isolated using TRIzol, followed by constructing small RNA libraries. And then, the qualified libraries were sequenced with the HiSeq 2500 SE50 system. The obtained clean reads were analyzed to identify conserved and novel miRNAs, and evaluate the expression, and also predict the target genes. The differential expressions of miRNAs were confirmed by RT-PCR.

Results

In this study, allotetraploid hybrid fish (4nAT) and diploi

SUBMITTER: Zhou R 

PROVIDER: S-EPMC4607245 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature