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Oscillating high-aspect-ratio monolithic silicon nanoneedle array enables efficient delivery of functional bio-macromolecules into living cells.

ABSTRACT: Delivery of biomolecules with use of nanostructures has been previously reported. However, both efficient and high-throughput intracellular delivery has proved difficult to achieve. Here, we report a novel material and device for the delivery of biomacromolecules into live cells. We attribute the successful results to the unique features of the system, which include high-aspect-ratio, uniform nanoneedles laid across a 2D array, combined with an oscillatory feature, which together allow rapid, forcible and efficient insertion and protein release into thousands of cells simultaneously.

SUBMITTER: Matsumoto D

PROVIDER: S-EPMC4607922 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature

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Oscillating high-aspect-ratio monolithic silicon nanoneedle array enables efficient delivery of functional bio-macromolecules into living cells.

Matsumoto Daisuke D Rao Sathuluri Ramachandra R Kato Yoshio Y Silberberg Yaron R YR Kawamura Ryuzo R Iwata Futoshi F Kobayashi Takeshi T Nakamura Chikashi C

Scientific reports 20151016

Delivery of biomolecules with use of nanostructures has been previously reported. However, both efficient and high-throughput intracellular delivery has proved difficult to achieve. Here, we report a novel material and device for the delivery of biomacromolecules into live cells. We attribute the successful results to the unique features of the system, which include high-aspect-ratio, uniform nanoneedles laid across a 2D array, combined with an oscillatory feature, which together allow rapid, fo ...[more]

PMID: 26471006

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