Functional characterization of an invertase inhibitor gene involved in sucrose metabolism in tomato fruit.
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ABSTRACT:
SUBMITTER: Zhang N
PROVIDER: S-EPMC4609536 | biostudies-literature | 2015 Oct
REPOSITORIES: biostudies-literature
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Functional characterization of an invertase inhibitor gene involved in sucrose metabolism in tomato fruit.
Zhang Ning N Jiang Jing J Yang Yan-li YL Wang Zhi-he ZH
Journal of Zhejiang University. Science. B 20151001 10
In this study, we produced tomato plants overexpressing an invertase inhibitor gene (Sly-INH) from tomato, using a simple and efficient transient transformation system. Compared with control plants, the expression of Sly-INH was highly upregulated in Sly-INH overexpressing plants, as indicated by real-time polymerase chain reaction (PCR). Physiological analysis revealed that Sly-INH inhibited the activity of cell wall invertase (CWIN), which increased sugar accumulation in tomato fruit. Furtherm ...[more]
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