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Genetic analysis of G12P[8] rotaviruses detected in the largest U.S. G12 genotype outbreak on record.


ABSTRACT: In 2006-07, 77 cases of gastroenteritis in Rochester, NY, USA were associated with rotavirus genotype G12P[8]. Sequence analysis identified a high degree of genetic relatedness among the VP7 and VP4 genes of the Rochester G12P[8] strains and between these strains and currently circulating human G12P[8] strains. Out of 77 samples, two and seven unique nucleotide sequences were identified for VP7 and VP4 genes, respectively. Rochester strain VP7 genes were found to occupy the G12-III lineage and VP4 genes clustered within the P[8]-3 lineage. Six strains contained non-synonymous nucleotide substitutions that produced amino acid changes at 6 sites in the VP8(?) region of the VP4 gene. Two sites (amino acids 242 and 246) were located in or near a described trypsin cleavage site. Selection analyses identified one positively selected VP7 site (107) and strong purifying selection at 58 sites within the VP7 gene as well as 2 of the 6 variant sites (79 and 218) in VP4.

SUBMITTER: Mijatovic-Rustempasic S 

PROVIDER: S-EPMC4612336 | biostudies-literature | 2014 Jan

REPOSITORIES: biostudies-literature

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Genetic analysis of G12P[8] rotaviruses detected in the largest U.S. G12 genotype outbreak on record.

Mijatovic-Rustempasic Slavica S   Teel Elizabeth N EN   Kerin Tara K TK   Hull Jennifer J JJ   Roy Sunando S   Weinberg Geoffrey A GA   Payne Daniel C DC   Parashar Umesh D UD   Gentsch Jon R JR   Bowen Michael D MD  

Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases 20131121


In 2006-07, 77 cases of gastroenteritis in Rochester, NY, USA were associated with rotavirus genotype G12P[8]. Sequence analysis identified a high degree of genetic relatedness among the VP7 and VP4 genes of the Rochester G12P[8] strains and between these strains and currently circulating human G12P[8] strains. Out of 77 samples, two and seven unique nucleotide sequences were identified for VP7 and VP4 genes, respectively. Rochester strain VP7 genes were found to occupy the G12-III lineage and V  ...[more]

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