Ontology highlight
ABSTRACT:
SUBMITTER: Yamamoto TM
PROVIDER: S-EPMC4612739 | biostudies-literature | 2014
REPOSITORIES: biostudies-literature
Yamamoto Tomomi M TM Wang Ling L Fisher Laura A LA Eckerdt Frank D FD Peng Aimin A
Cell cycle (Georgetown, Tex.) 20140101 22
Greatwall (Gwl) functions as an essential mitotic kinase by antagonizing protein phosphatase 2A. In this study we identified Hsp90, Cdc37 and members of the importin α and β families as the major binding partners of Gwl. Both Hsp90/Cdc37 chaperone and importin complexes associated with the N-terminal kinase domain of Gwl, whereas an intact glycine-rich loop at the N-terminus of Gwl was essential for binding of Hsp90/Cdc37 but not importins. We found that Hsp90 inhibition led to destabilization o ...[more]