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The centriolar protein CPAP G-box: an amyloid fibril in a single domain.


ABSTRACT: Centrioles are evolutionarily conserved cylindrical cell organelles with characteristic radial symmetry. Despite their considerable size (400 nm × 200 nm, in humans), genetic studies suggest that relatively few protein components are involved in their assembly. We recently characterized the molecular architecture of the centrosomal P4.1-associated protein (CPAP), which is crucial for controlling the centriolar cylinder length. Here, we review the remarkable architecture of the C-terminal domain of CPAP, termed the G-box, which comprises a single, entirely solvent exposed, antiparallel ?-sheet. Molecular dynamics simulations support the stability of the G-box domain even in the face of truncations or amino acid substitutions. The similarity of the G-box domain to amyloids (or amyloid precursors) is strengthened by its oligomeric arrangement to form continuous fibrils. G-box fibrils were observed in crystals as well as in solution and are also supported by simulations. We conclude that the G-box domain may well represent the best analogue currently available for studies of exposed ?-sheets, unencumbered by additional structural elements or severe aggregations problems.

SUBMITTER: Cutts EE 

PROVIDER: S-EPMC4613516 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature

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The centriolar protein CPAP G-box: an amyloid fibril in a single domain.

Cutts Erin E EE   Inglis Alison A   Stansfeld Phillip J PJ   Vakonakis Ioannis I   Hatzopoulos Georgios N GN  

Biochemical Society transactions 20151001 5


Centrioles are evolutionarily conserved cylindrical cell organelles with characteristic radial symmetry. Despite their considerable size (400 nm × 200 nm, in humans), genetic studies suggest that relatively few protein components are involved in their assembly. We recently characterized the molecular architecture of the centrosomal P4.1-associated protein (CPAP), which is crucial for controlling the centriolar cylinder length. Here, we review the remarkable architecture of the C-terminal domain  ...[more]

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