Poly(ADP-ribosyl)ation is recognized by ECT2 during mitosis.
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ABSTRACT:
SUBMITTER: Li M
PROVIDER: S-EPMC4614318 | biostudies-literature | 2014
REPOSITORIES: biostudies-literature
Publications
Poly(ADP-ribosyl)ation is recognized by ECT2 during mitosis.
Li Mo M Bian Chunjing C Yu Xiaochun X
Cell cycle (Georgetown, Tex.) 20140101 18
Poly(ADP-ribosyl)ation is an unique posttranslational modification and required for spindle assembly and function during mitosis. However, the molecular mechanism of poly(ADP-ribose) (PAR) in mitosis remains elusive. Here, we show the evidence that PAR is recognized by ECT2, a key guanine nucleotide exchange factor in mitosis. The BRCT domain of ECT2 directly binds to PAR both in vitro and in vivo. We further found that α-tubulin is PARylated during mitosis. PARylation of α-tubulin is recognized ...[more]
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