Ontology highlight
ABSTRACT:
SUBMITTER: Reinhardt K
PROVIDER: S-EPMC4614475 | biostudies-literature | 2015 Sep
REPOSITORIES: biostudies-literature
Journal of the Royal Society, Interface 20150901 110
We explore the possibility of characterizing sperm cells without the need to stain them using spectral and fluorescence lifetime analyses after multi-photon excitation in an insect model. The autofluorescence emission spectrum of sperm of the common bedbug, Cimex lectularius, was consistent with the presence of flavins and NAD(P)H. The mean fluorescence lifetimes showed smaller variation in sperm extracted from the male (tau m, τm = 1.54-1.84 ns) than in that extracted from the female sperm stor ...[more]