Ontology highlight
ABSTRACT:
SUBMITTER: Li CL
PROVIDER: S-EPMC4617536 | biostudies-literature | 2015 Sep
REPOSITORIES: biostudies-literature
Li Chia-Lung CL Golebiowski Filip M FM Onishi Yuki Y Samara Nadine L NL Sugasawa Kaoru K Yang Wei W
Molecular cell 20150901 6
Transcription factor IIH (TFIIH) is essential for both transcription and nucleotide excision repair (NER). DNA lesions are initially detected by NER factors XPC and XPE or stalled RNA polymerases, but only bulky lesions are preferentially repaired by NER. To elucidate substrate specificity in NER, we have prepared homogeneous human ten-subunit TFIIH and its seven-subunit core (Core7) without the CAK module and show that bulky lesions in DNA inhibit the ATPase and helicase activities of both XPB ...[more]