Ontology highlight
ABSTRACT:
SUBMITTER: Yu H
PROVIDER: S-EPMC4618232 | biostudies-literature | 2015 Nov
REPOSITORIES: biostudies-literature
Yu Hongjun H Takeuchi Megumi M LeBarron Jamie J Kantharia Joshua J London Erwin E Bakker Hans H Haltiwanger Robert S RS Li Huilin H Takeuchi Hideyuki H
Nature chemical biology 20150928 11
A major question remaining in glycobiology is how a glycosyltransferase (GT) that retains the anomeric linkage of a sugar catalyzes the reaction. Xyloside α-1,3-xylosyltransferase (XXYLT1) is a retaining GT that regulates Notch receptor activation by adding xylose to the Notch extracellular domain. Here, using natural acceptor and donor substrates and active Mus musculus XXYLT1, we report a series of crystallographic snapshots along the reaction, including an unprecedented natural and competent ...[more]