Use of Nanostructure-Initiator Mass Spectrometry to Deduce Selectivity of Reaction in Glycoside Hydrolases.
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ABSTRACT:
SUBMITTER: Deng K
PROVIDER: S-EPMC4621489 | biostudies-literature | 2015
REPOSITORIES: biostudies-literature
Publications
Use of Nanostructure-Initiator Mass Spectrometry to Deduce Selectivity of Reaction in Glycoside Hydrolases.
Deng Kai K Takasuka Taichi E TE Bianchetti Christopher M CM Bergeman Lai F LF Adams Paul D PD Northen Trent R TR Fox Brian G BG
Frontiers in bioengineering and biotechnology 20151027
Chemically synthesized nanostructure-initiator mass spectrometry (NIMS) probes derivatized with tetrasaccharides were used to study the reactivity of representative Clostridium thermocellum β-glucosidase, endoglucanases, and cellobiohydrolase. Diagnostic patterns for reactions of these different classes of enzymes were observed. Results show sequential removal of glucose by the β-glucosidase and a progressive increase in specificity of reaction from endoglucanases to cellobiohydrolase. Time-depe ...[more]
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