Dataset Information

DNA Barcoding of Neotropical Sand Flies (Diptera, Psychodidae, Phlebotominae): Species Identification and Discovery within Brazil.

ABSTRACT: DNA barcoding has been an effective tool for species identification in several animal groups. Here, we used DNA barcoding to discriminate between 47 morphologically distinct species of Brazilian sand flies. DNA barcodes correctly identified approximately 90% of the sampled taxa (42 morphologically distinct species) using clustering based on neighbor-joining distance, of which four species showed comparatively higher maximum values of divergence (range 4.23-19.04%), indicating cryptic diversity. The DNA barcodes also corroborated the resurrection of two species within the shannoni complex and provided an efficient tool to differentiate between morphologically indistinguishable females of closely related species. Taken together, our results validate the effectiveness of DNA barcoding for species identification and the discovery of cryptic diversity in sand flies from Brazil.

SUBMITTER: Pinto Ide S

PROVIDER: S-EPMC4624639 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature

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DNA Barcoding of Neotropical Sand Flies (Diptera, Psychodidae, Phlebotominae): Species Identification and Discovery within Brazil.

Pinto Israel de Souza Ide S Chagas Bruna Dias das BD Rodrigues Andressa Alencastre Fuzari AA Ferreira Adelson Luiz AL Rezende Helder Ricas HR Bruno Rafaela Vieira RV Falqueto Aloisio A Andrade-Filho José Dilermando JD Galati Eunice Aparecida Bianchi EA Shimabukuro Paloma Helena Fernandes PH Brazil Reginaldo Peçanha RP Peixoto Alexandre Afranio AA

PloS one 20151027 10

DNA barcoding has been an effective tool for species identification in several animal groups. Here, we used DNA barcoding to discriminate between 47 morphologically distinct species of Brazilian sand flies. DNA barcodes correctly identified approximately 90% of the sampled taxa (42 morphologically distinct species) using clustering based on neighbor-joining distance, of which four species showed comparatively higher maximum values of divergence (range 4.23-19.04%), indicating cryptic diversity. ...[more]

PMID: 26506007

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Project description:Sand fly species are traditionally identified using morphological traits, though this method is hampered by the presence of cryptic species. DNA barcoding is a widely used tool in the case of insects of medical importance, where it is necessary to know quickly which species are present in a transmission area. Here, we assess the usefulness of mitochondrial cytochrome c oxidase subunit I (COI) DNA barcoding as a practical tool for species identification, correct assignment of isomorphic females, and to evaluate the detection of cryptic diversity that occurs in the same species. A fragment of the COI gene was used to generate 156 new barcode sequences for sand flies from different countries of the Neotropical region, mainly Colombia, which had been identified morphologically as 43 species. The sequencing of the COI gene allowed the detection of cryptic diversity within species and correctly associated isomorphic females with males identified by morphology. The maximum intraspecific genetic distances ranged from 0 to 8.32% and 0 to 8.92% using uncorrected p distances and the Kimura 2-parameter (K2P) model, respectively. The minimum interspecific distance (nearest neighbor) for each species ranged from 1.5 to 14.14% and 1.51 to 15.7% using p and K2P distances, respectively. Three species had more than 3% maximum intraspecific distance: Psychodopygus panamensis, Micropygomyia cayennensis cayennensis, and Pintomyia evansi. They also were split into at least two molecular operational taxonomic units (MOTUs) each, using different species delimitation algorithms. Regarding interspecific genetic distances, the species of the genera Nyssomyia and Trichophoromyia generated values lower than 3% (except Nyssomyia ylephiletor and Ny. trapidoi). However, the maximum intraspecific distances did not exceed these values, indicating the presence of a barcode gap despite their proximity. Also, nine sand fly species were DNA barcoded for the first time: Evandromyia georgii, Lutzomyia sherlocki, Ny. ylephiletor, Ny. yuilli pajoti, Psathyromyia punctigeniculata, Sciopemyia preclara, Trichopygomyia triramula, Trichophoromyia howardi, and Th. velezbernali. The COI DNA barcode analysis enabled the correct delimitation of several Neotropical sand fly species from South and Central America and raised questions about the presence of cryptic species for some taxa, which should be further assessed.

Project description:BackgroundSandflies are insects important for the transmission cycles of the leishmaniases. Despite being studied since the 1960s in the State of Rondônia (Brazil), several gaps exist regarding our working knowledge of these insects. This study aimed to construct an up-to-date database of sandflies using complementary information from the speciesLink database and the scientific literature, as well as to elaborate integrated abundance maps. We identified 153,155 records of sandflies captured in Rondônia between 1965-2021; after exclusion, 147,258 reports (speciesLink - 3,408, Rondônia studies - 143,850) associated with 15 genera and 140 species were mapped. The most abundant species observed were Psychodopygusdavisi (Root, 1934) (43,818 records), Nyssomyiawhitmani (Antunes & Coutinho, 1939) (12,594), Psychodopyguscarrerai (Barretto, 1946) (11,840), Psychodopygushirsutus (Mangabeira, 1942) (9,676), Nyssomyiaantunesi (Coutinho, 1939) (8,847), Trichophoromyiaubiquitalis (Mangabeira, 1942) (5,505), Psychodopygusgeniculatus (Mangabeira, 1941) (4,644), Pintomyianevesi (Damasceno & Arouck, 1956) (4,140), Trichophoromyiaauraensis (Mangabeira, 1942) (3,579), Psychodopyguscomplexus (Mangabeira, 1941) (2,659), Nyssomyiafraihai (Martins, Falcão & Silva, 1979) (2,504) and Bichromomyiaflaviscutellata (Mangabeira, 1942) (1,418). A total of 20 records of Leishmania detection corresponded to eight sand fly species. The present dataset provides updated information on the distribution of sandflies of Rondônia, including those considered potential vectors of Leishmania, which should prove useful to guide future studies.New informationThe present study provides an extensive dataset built from all studies reporting phlebotomine sandflies in the Brazilian State of Rondônia. Online distribution maps can aid scientists who wish to consult the updated list of sand fly species and view the distribution of these insects, as well as those considered potential vectors of Leishmania. The results of the present study can serve as the basis for future studies on sandflies conducted in the State.

Project description:BackgroundThe distribution of phlebotomine sand flies is changing rapidly due to climate change. This issue has implications for the epidemiology of sand fly-borne diseases, especially sand fly-associated viruses. Few studies concerning sand fly-associated viruses have been conducted in Thailand. Therefore, this study aimed to perform a molecular survey of groups of pathogenic RNA viruses belonging to the Orbivirus, Phlebovirus, and Flavivirus genera and family Rhabdoviridae in sand fly samples collected from southern Thailand.MethodsSand flies were collected at two locations in Trang and Songkhla provinces of southern Thailand, and individual sand fly samples were processed for species identification and virus detection. The Orbivirus, Phlebovirus, and Flavivirus genera and family Rhabdoviridae molecular determination was performed by RT-PCR, and positive samples were identified by cloning and sequencing, cell culture inoculation, and phylogenetic analysis.ResultsThe results presented in this study were based on the analysis of a total of 331 female sand flies. This molecular study revealed evidence of Rhabdoviridae family virus presence in Phlebotomus papatasi (3/331, 0.9%). The findings demonstrated a new cluster of rhabdovirus that was closely related to Bactrocera dorsalis sigmavirus strain BDSV.abc5 and the lineages of insect-specific Rhabdoviridae. In addition, the Bayesian tree suggested that the common ancestor of this group was the dimarhabdovirus clade. It was assumed that the virus may have switched hosts during its evolution. However, the detection of Orbivirus, Phlebovirus, and Flavivirus genera using specific primers for RT-PCR was negative in the collected sand flies.ConclusionsThere is limited knowledge on the genetic diversity and ecology of Rhabdoviridae in Thailand. This is the first data regarding the circulation of Rhabdoviridae in Ph. papatasi from Thailand. We found a new cluster of rhabdoviruses that was close to the new B. dorsalis sigmavirus. It is possible that there is a great deal of diversity in this family yet to be discovered, and a more extensive survey for new rhabdoviruses may uncover viruses from a wide diversity of host taxa and broaden our understanding of the relationships among the Rhabdoviridae.

Dataset Information

DNA Barcoding of Neotropical Sand Flies (Diptera, Psychodidae, Phlebotominae): Species Identification and Discovery within Brazil.

Publications

DNA Barcoding of Neotropical Sand Flies (Diptera, Psychodidae, Phlebotominae): Species Identification and Discovery within Brazil.

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