Ontology highlight
ABSTRACT:
SUBMITTER: Klinger M
PROVIDER: S-EPMC4624875 | biostudies-literature | 2015
REPOSITORIES: biostudies-literature
Klinger Mark M Pepin Francois F Wilkins Jen J Asbury Thomas T Wittkop Tobias T Zheng Jianbiao J Moorhead Martin M Faham Malek M
PloS one 20151028 10
Monitoring antigen-specific T cells is critical for the study of immune responses and development of biomarkers and immunotherapeutics. We developed a novel multiplex assay that combines conventional immune monitoring techniques and immune receptor repertoire sequencing to enable identification of T cells specific to large numbers of antigens simultaneously. We multiplexed 30 different antigens and identified 427 antigen-specific clonotypes from 5 individuals with frequencies as low as 1 per mil ...[more]