Dataset Information

The receptor-like kinase SOBIR1 interacts with Brassica napus LepR3 and is required for Leptosphaeria maculans AvrLm1-triggered immunity.

ABSTRACT: The fungus Leptosphaeria maculans (L. maculans) is the causal agent of blackleg disease of canola/oilseed rape (Brassica napus) worldwide. We previously reported cloning of the B. napus blackleg resistance gene, LepR3, which encodes a receptor-like protein. LepR3 triggers localized cell death upon recognition of its cognate Avr protein, AvrLm1. Here, we exploited the Nicotiana benthamiana model plant to investigate the recognition mechanism of AvrLm1 by LepR3. Co-expression of the LepR3/AvrLm1 gene pair in N. benthamiana resulted in development of a hypersensitive response (HR). However, a truncated AvrLm1 lacking its indigenous signal peptide was compromised in its ability to induce LepR3-mediated HR, indicating that AvrLm1 is perceived by LepR3 extracellularly. Structure-function analysis of the AvrLm1 protein revealed that the C-terminal region of AvrLm1 was required for LepR3-mediated HR in N. benthamiana and for resistance to L. maculans in B. napus. LepR3 was shown to be physically interacting with the B. napus receptor like kinase, SOBIR1 (BnSOBIR1). Silencing of NbSOBIR1 or NbSERK3 (BAK1) compromised LepR3-AvrLm1-dependent HR in N. benthamiana, suggesting that LepR3-mediated resistance to L. maculans in B. napus requires SOBIR1 and BAK1/SERK3. Using this model system, we determined that BnSOBIR1 and SERK3/BAK1 are essential partners in the LepR3 signaling complex and were able to define the AvrLm1 effector domain.

SUBMITTER: Ma L

PROVIDER: S-EPMC4625043 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature

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The receptor-like kinase SOBIR1 interacts with Brassica napus LepR3 and is required for Leptosphaeria maculans AvrLm1-triggered immunity.

Ma Lisong L Borhan M Hossein MH

Frontiers in plant science 20151029

The fungus Leptosphaeria maculans (L. maculans) is the causal agent of blackleg disease of canola/oilseed rape (Brassica napus) worldwide. We previously reported cloning of the B. napus blackleg resistance gene, LepR3, which encodes a receptor-like protein. LepR3 triggers localized cell death upon recognition of its cognate Avr protein, AvrLm1. Here, we exploited the Nicotiana benthamiana model plant to investigate the recognition mechanism of AvrLm1 by LepR3. Co-expression of the LepR3/AvrLm1 g ...[more]

PMID: 26579176

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Project description:While our understanding of the genetics underlying the Brassica-Leptosphaeria pathosystem has advanced greatly in the last decade, differences in molecular responses due to interaction between resistance genes and host genetic background has not been studied. We applied RNAseq technology to monitor the transcriptome profiles of Brassica napus (Bn) lines carrying one of four blackleg R genes (Rlm2, Rlm3, LepR1 & LepR2) in Topas or Westar background, during the early stages of infection by a Leptosphaeria maculans (Lm) isolate carrying the corresponding Avr genes. We observed upregulation of host genes involved in hormone signalling, cell wall thickening, response to chitin and glucosinolate production in all R gene lines at 3 day after inoculation (dai) albeit having higher level of expression in LepR1 and Rlm2 than in Rlm3 and LepR2 lines. Bn-SOBIR1 (Suppressor Of BIR1-1), a receptor like kinase (RLK) that forms complex receptor like proteins (RLPs) was highly expressed in LepR1 and Rlm2 at 3 dai. In contrast Bn-SOBIR1 induction was low in Rlm3 line, which could indicate that Rlm3 may function independent of SOBIR1. Expression of Salicylic acid (SA) related defense was enhanced in LepR1 and Rlm2 at 3 dai. In contrast to SA, expression of Bn genes with homology to PDF1.2, a jasmonic acid (JA) pathway marker, were increased in all Rlm and LepR lines at 6 and 9 dai. Effect of host genetic background on induction of defense, was determined by comparison of LepR1 and LepR2 in Topas vs Westar genotype (i.e. T-LepR1 vs W-LepR1 and T-LepR2 vs W-LepR2). In both cases (regardless of R gene) overall number of defense related genes at the earliest time point (3 dai) was higher in Tops compared to Westar. SA and JA markers genes such as PR1 and PDF1.2 were more induced in Topas compared to Westar introgression lines at this time point. Even in the absence of any R gene, effect of Topas genotype in enhanced defense, was also evident by the induction of PDF1.2 that started at a low level at 3 dai and peaked at 6 and 9 dai, while no induction in Westar genotype was observed at any of these time points. Overall, variation in time and intensity of expression of genes related to defense, was clearly dependent on both R gene and the host genotype.

Dataset Information

The receptor-like kinase SOBIR1 interacts with Brassica napus LepR3 and is required for Leptosphaeria maculans AvrLm1-triggered immunity.

Publications

The receptor-like kinase SOBIR1 interacts with Brassica napus LepR3 and is required for Leptosphaeria maculans AvrLm1-triggered immunity.

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