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Differential temporal changes of primary and secondary bacterial symbionts and whitefly host fitness following antibiotic treatments.

ABSTRACT: Where multiple symbionts coexist in the same host, the selective elimination of a specific symbiont may enable the roles of a given symbiont to be investigated. We treated the Mediterranean species of the whitefly Bemisia tabaci complex by oral delivery of the antibiotic rifampicin, and then examined the temporal changes of its primary symbiont "Candidatus Portiera aleyrodidarum" and secondary symbiont "Ca. Hamiltonella defensa" as well as host fitness for three generations. In adults treated with rifampicin (F0), the secondary symbiont was rapidly reduced, approaching complete disappearance as adults aged. In contrast, the primary symbiont was little affected until later in the adult life. In the offspring of these adults (F1), both symbionts were significantly reduced and barely detectable when the hosts reached the adult stage. The F1 adults laid few eggs (F2), all of which failed to hatch. Mating experiments illustrated that the negative effects of rifampicin on host fitness were exerted via female hosts but not males. This study provides the first evidence of differential temporal reductions of primary and secondary symbionts in whiteflies following an antibiotic treatment. Studies that disrupt functions of bacterial symbionts must consider their temporal changes.

SUBMITTER: Zhang CR

PROVIDER: S-EPMC4625128 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature

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Differential temporal changes of primary and secondary bacterial symbionts and whitefly host fitness following antibiotic treatments.

Zhang Chang-Rong CR Shan Hong-Wei HW Xiao Na N Zhang Fan-Di FD Wang Xiao-Wei XW Liu Yin-Quan YQ Liu Shu-Sheng SS

Scientific reports 20151029

Where multiple symbionts coexist in the same host, the selective elimination of a specific symbiont may enable the roles of a given symbiont to be investigated. We treated the Mediterranean species of the whitefly Bemisia tabaci complex by oral delivery of the antibiotic rifampicin, and then examined the temporal changes of its primary symbiont "Candidatus Portiera aleyrodidarum" and secondary symbiont "Ca. Hamiltonella defensa" as well as host fitness for three generations. In adults treated wi ...[more]

PMID: 26510682

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Dataset Information

Differential temporal changes of primary and secondary bacterial symbionts and whitefly host fitness following antibiotic treatments.

Publications

Differential temporal changes of primary and secondary bacterial symbionts and whitefly host fitness following antibiotic treatments.

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