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By the company they keep: interaction networks define the binding ability of transcription factors.

ABSTRACT: Access to genome-wide data provides the opportunity to address questions concerning the ability of transcription factors (TFs) to assemble in distinct macromolecular complexes. Here, we introduce the PAnDA (Protein And DNA Associations) approach to characterize DNA associations with human TFs using expression profiles, protein-protein interactions and recognition motifs. Our method predicts TF binding events with >0.80 accuracy revealing cell-specific regulatory patterns that can be exploited for future investigations. Even when the precise DNA-binding motifs of a specific TF are not available, the information derived from protein-protein networks is sufficient to perform high-confidence predictions (area under the ROC curve of 0.89). PAnDA is freely available at http://service.tartaglialab.com/new_submission/panda.

SUBMITTER: Cirillo D

PROVIDER: S-EPMC4627061 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature

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By the company they keep: interaction networks define the binding ability of transcription factors.

Cirillo Davide D Botta-Orfila Teresa T Tartaglia Gian Gaetano GG

Nucleic acids research 20150618 19

Access to genome-wide data provides the opportunity to address questions concerning the ability of transcription factors (TFs) to assemble in distinct macromolecular complexes. Here, we introduce the PAnDA (Protein And DNA Associations) approach to characterize DNA associations with human TFs using expression profiles, protein-protein interactions and recognition motifs. Our method predicts TF binding events with >0.80 accuracy revealing cell-specific regulatory patterns that can be exploited fo ...[more]

PMID: 26089389

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