A perfusion-capable microfluidic bioreactor for assessing microbial heterologous protein production.
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ABSTRACT:
SUBMITTER: Mozdzierz NJ
PROVIDER: S-EPMC4627644 | biostudies-literature | 2015 Jul
REPOSITORIES: biostudies-literature
Publications
A perfusion-capable microfluidic bioreactor for assessing microbial heterologous protein production.
Mozdzierz Nicholas J NJ Love Kerry R KR Lee Kevin S KS Lee Harry L T HL Shah Kartik A KA Ram Rajeev J RJ Love J Christopher JC
Lab on a chip 20150701 14
We present an integrated microfluidic bioreactor for fully continuous perfusion cultivation of suspended microbial cell cultures. This system allowed continuous and stable heterologous protein expression by sustaining the cultivation of Pichia pastoris over 11 days. This technical capability also allowed testing the impact of perfusion conditions on protein expression. This advance should enable small-scale models for process optimization in continuous biomanufacturing. ...[more]
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