Unknown

Dataset Information

0

The zebrafish/tumor xenograft angiogenesis assay as a tool for screening anti-angiogenic miRNAs.


ABSTRACT: The zebrafish/tumor xenograft angiogenesis assay is used to approach tumor angiogenesis, a pivotal step in cancer progression and target for anti-tumor therapies. Here, we evaluated whether the assay could allow the identification of microRNAs having an anti-angiogenic potential. For that, we transfected DU-145 prostate cancer cells with four microRNAs (miR-125a, miR-320, miR-487b, miR-492) responsive to both anti- and pro-angiogenic stimuli applied to human umbilical vein endothelial cells. After transfection, DU-145 cells were injected close to the developing subintestinal vessels of transgenic Tg(Kdrl:eGFP)s843 zebrafish embryos that express green fluorescent protein under the control of Kdrl promoter. At 72 h post-fertilization, we observed that green fluorescent protein-positive neo-vessels infiltrated the graft of DU-145 transfected with miR-125a, miR-320, and miR-487b. Vice versa, neo-vessel formation and tumor cell infiltration were inhibited when DU-145 cells transfected with miR-492 were used. These results indicated that the zebrafish/tumor xenograft assay was adequate to identify microRNAs able to suppress the release of angiogenic growth factors by angiogenic tumor cells.

SUBMITTER: Chiavacci E 

PROVIDER: S-EPMC4628928 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature

altmetric image

Publications

The zebrafish/tumor xenograft angiogenesis assay as a tool for screening anti-angiogenic miRNAs.

Chiavacci Elena E   Rizzo Milena M   Pitto Letizia L   Patella Francesca F   Evangelista Monica M   Mariani Laura L   Rainaldi Giuseppe G  

Cytotechnology 20140620 6


The zebrafish/tumor xenograft angiogenesis assay is used to approach tumor angiogenesis, a pivotal step in cancer progression and target for anti-tumor therapies. Here, we evaluated whether the assay could allow the identification of microRNAs having an anti-angiogenic potential. For that, we transfected DU-145 prostate cancer cells with four microRNAs (miR-125a, miR-320, miR-487b, miR-492) responsive to both anti- and pro-angiogenic stimuli applied to human umbilical vein endothelial cells. Aft  ...[more]

Similar Datasets

| S-EPMC6019900 | biostudies-other
| S-EPMC7370283 | biostudies-literature
| S-EPMC7020037 | biostudies-literature
| S-EPMC3508273 | biostudies-literature
| S-EPMC8328589 | biostudies-literature
| S-EPMC6234738 | biostudies-literature
| S-EPMC4632735 | biostudies-literature
| S-EPMC5795993 | biostudies-literature
2016-11-07 | PXD005217 | Pride
| S-EPMC9330857 | biostudies-literature